Determination and differentiation of the potato cyst nematodes Globodera rostochiensis and Globodera pallida by PCR
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چکیده
منابع مشابه
The effect of linalool on second-stage juveniles of the potato cyst nematodes Globodera rostochiensis and G. pallida.
Linalool is either a toxic compound to a few species of plant parasitic nematodes or attractive to entomopathogenic nematodes. This compound is produced and emitted by several host plants of Globodera rostochiensis and G. pallida, the potato cyst nematodes (PCN). With the aim to reveal the effect of linalool on PCN, laboratory assays were carried out. Survival of PCN second-stage juveniles (J2s...
متن کاملProbability of Globodera rostochiensis Spread on Equipment and Potato Tubers.
The probability of spreading cysts of Globodera rostochiensis on farming equipment and potato tubers was investigated in naturally infested field plots. The number of cysts recovered from soil that adhered to equipment differed significantly between different pieces of equipment. These differences were related to initial nematode density and, in most cases, to the volume of soil that adhered to...
متن کاملEarly responses of resistant and susceptible potato roots during invasion by the potato cyst nematode Globodera rostochiensis.
Signals from roots of resistant (cv. Maris Piper) and susceptible (cv. Désirée) potato cultivars during invasion by second stage juveniles (J2s) of the potato cyst nematode, Globodera rostochiensis, were investigated. Novel experimental chambers enabled the recording of electrophysiological responses from roots during nematode invasion. The root cell membrane potentials were maintained througho...
متن کاملField evaluation of the nematicide fluensulfone for control of the potato cyst nematode Globodera pallida
BACKGROUND Three field experiments evaluated the performance of the nematicide fluensulfone against the potato cyst nematode Globodera pallida in Shropshire, England. RESULTS Experiments 1 and 2 showed reduced root infection and lowered multiplication of G. pallida following fluensulfone (Nimitz 15G(®) ) soil treatments at five rates (1.95, 3.00, 4.05 (full rate), 5.05 and 6.00 kg AI ha(-1) )...
متن کاملStage-specific Monoclonal Antibodies to the Potato Cyst Nematode Globodera pallida Stone (Behrens).
Using standard hybridoma technology and hierarchical screening, monoclonal antibodies (MAbs) were obtained with specific reactivity against two developmental stages of Globodera pallida. The procedure was based on enzyme-linked immunosorbent assay (ELISA) with homogenates prepared from second-stage juveniles, young adult females, and potato roots. Hybridomas were formed by fusing myelomas with ...
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ژورنال
عنوان ژورنال: Plant Protection Science
سال: 2000
ISSN: 1212-2580,1805-9341
DOI: 10.17221/9628-pps